Cells, Free Full-Text

Cancer stem cells play a crucial role in tumor initiation, metastasis, and resistance to treatment. Cellular heterogeneity and plasticity complicate the isolation of cancer stem cells. The impact of intra-tumor cellular heterogeneity using a label-free approach remains understudied in the context of treatment resistance. Here, we use the sedimentation field–flow fractionation technique to separate, without labeling, cell subpopulations of colorectal cancer cell lines and primary cultures according to their biophysical properties. One of the three sorted cell subpopulations exhibits characteristics of cancer stem cells, including high tumorigenicity in vivo and a higher frequency of tumor-initiating cells compared to the other subpopulations. Due to its chemoresistance, two- and three-dimensional in vitro chemosensitivity assays highlight the therapeutic relevance of this cancer stem cell subpopulation. Thus, our results reveal the major implication of intra-tumor cellular heterogeneity, including cancer stem cells in treatment resistance, thanks to our label-free cell sorting approach. This approach enables—by breaking down the tumor—the study the individualized response of each sorted tumor cell subpopulation and to identify chemoresistance, thus offering new perspectives for personalized therapy.

Cells, Free Full-Text

Continuous-Flow and Continuous-Exchange Cell-Free Translation

HIV-1 cell-to-cell transmission and broadly neutralizing

THE LIVES OF A CELL : LEWIS THOMAS : Free Download, Borrow, and

Cells, Free Full-Text

Sequencing of Circulating Cell-free DNA during Pregnancy

Label-free, full-field visualization of red blood cell (RBC

Five-Year Outcomes for Refractory B-Cell Lymphomas with CAR T-Cell

Postbiotics-parabiotics: the new horizons in microbial biotherapy

Cell-Free DNA and Apoptosis: How Dead Cells Inform About the